Me and My Research

Brief introduction:

I'm a senior scientist based at Statens Serum Institut, Copenhagen, Denmark at the Department of Microbiology and Infection Control.

Together with excellent colleagues, I have in few years contributed significantly to what today is known about Blastocystis, an intestinal parasite of unknown clinical significance colonising/infecting hundreds of millions of people in both developed and developing countries. I have published about 55 seminal and review articles in peer-reviewed, international journals focussing entirely or partly on Blastocystis.

During my PhD I took the initiative to standardise Blastocystis terminology and evaluate diagnostic methods; I managed to cross correlate and interpret a vast pool of molecular data from different research labs. Thanks to these efforts, researchers across the globe now employ the same terminology for Blastocystis, and a clear picture of the relative distribution of different subtypes in various cohorts in various countries is now building up.

I have detected and delineated several new species (ribosomal lineages) of protistan genera such as Entamoeba, Iodamoeba and Blastocystis, and I was the first ever to report on Dientamoeba fragilis in Denmark.

Output from my research has provided incentive for improving routine diagnosis of intestinal parasites and has resulted in the implementation of novel, DNA-based methods for the routine screening of patients with diarrhoea for intestinal parasites of clinical relevance. My research has had a major impact on current guidelines for the detection of intestinal parasites and offers a critical approach to the implementation of novel technology in different fields of clinical microbiology.

I have been able to attract substantial funding by myself and in collaboration with colleagues.

Ongoing research in our lab also includes a randomised controlled treatment study of Dientamoeba fragilis infections in children in Denmark.

I have supervised dozens of Bachelor and MSc students, and co-supervised PhD students.

- Bachelor of Medicine (University of Aarhus, Denmark)
- MSc in Parasitology (University of Copenhagen, Denmark)
- PhD in Health Sciences (University of Copenhagen, Denmark)

- Field work: WHO, Vientiane, Lao PDR (2003)
- PhD training at Murdoch University, Western Australia (2006)
- Post doc studies at London School of Hygiene & Tropical Medicine, London, UK (2009-2012)

Key research interests:
- Parasitology
- Mycology
- Clinical Microbiology
- Infectious Diseases
- Gastroenterology

More than 130 papers in national and international peer-reviewed journals
First paper appeared in 2006
H-index: 43
M-index: 2.4

Grants obtained by myself:
2013: Brdr. Hartmanns Fond (Denmark), €12,000
2012: Lundbeck Foundation (Denmark), €200,000
2012: Marie Curie Career Reintegration Grant (EU), €100,000
2012: Danish Agency for Science Technology and Innovation, €15,000
2012: TRYG Fonden (Denmark), €46,000
2011: AP Møller Foundation (Denmark), €6,000
2009: Danish Agency for Science Technology and Innovation, €230,000

2013: Fritz Kauffmann Prize (Denmark), €10,000.

Extra-curricular activities:
- A history of several board memberships
- Associate Faculty Member of Faculty 1000.
- Reviewer for > 30 scientific journals, including CMR, JCM, IJP, Trends in Parasitology, Eurosurveillance.

Social media:
I've got an updated CV here.
Follow me on Twitter.
There is Facebook group on Blastocystis as well and the Blastocystis Parasite Blog also appears on Facebook.

ORCID # 0000-0002-1417-7048

Scientific Articles:

Items 1-152 of 152 (Display the 152 citations in PubMed)


Further Insight into the Genetic Diversity of Entamoeba coli and Entamoeba hartmanni.

Stensvold CR, Ascuña-Durand K, Chihi A, Belkessa S, Kurt Ö, El-Badry A, van der Giezen M, Clark G.

J Eukaryot Microbiol. 2022 Sep 28:e12949. doi: 10.1111/jeu.12949. Online ahead of print.

PMID: 36168968


Unravelling the Phylogeny of a Common Intestinal Protist: Intrageneric Diversity of Endolimax.

Hocke EF, Jamy M, Burki F, Clark CG, Stensvold CR.

Protist. 2022 Sep 7;173(5):125908. doi: 10.1016/j.protis.2022.125908. Online ahead of print.

PMID: 36152390


Strongyloides stercoralis hyperinfection syndrome with cerebral involvement.

Pedersen AA, Hartmeyer GN, Stensvold CR, Martin-Iguacel R.

BMJ Case Rep. 2022 Sep 22;15(9):e247032. doi: 10.1136/bcr-2021-247032.

PMID: 36137646 Free PMC article.


Anisakiasis erhvervet i Danmark.

Mejer N, Stensvold CR, Porskrog A, Bulut M.

Ugeskr Laeger. 2022 Jun 6;184(23):V71117.

PMID: 35703062 Danish. No abstract available.


Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans.

Šloufová M, Lhotská Z, Jirků M, Petrželková KJ, Stensvold CR, Cinek O, Pomajbíková KJ.

Parasite. 2022;29:30. doi: 10.1051/parasite/2022029. Epub 2022 May 31.

PMID: 35638752 Free PMC article.


Stool Microbiota Diversity Analysis of Blastocystis-Positive and Blastocystis-Negative Individuals.

Stensvold CR, Sørland BA, Berg RPKD, Andersen LO, van der Giezen M, Bowtell JL, El-Badry AA, Belkessa S, Kurt Ö, Nielsen HV.

Microorganisms. 2022 Jan 31;10(2):326. doi: 10.3390/microorganisms10020326.

PMID: 35208781 Free PMC article.


Amplicon-based next-generation sequencing of eukaryotic nuclear ribosomal genes (metabarcoding) for the detection of single-celled parasites in human faecal samples.

Chihi A, O'Brien Andersen L, Aoun K, Bouratbine A, Stensvold CR.

Parasite Epidemiol Control. 2022 Jan 30;17:e00242. doi: 10.1016/j.parepi.2022.e00242. eCollection 2022 May.

PMID: 35146142 Free PMC article.


Human subcutaneous dirofilariasis: the 'migrating' skin tumor.

Mistry MA, Hoejvig J, Helleberg M, Stensvold CR, Jokelainen P, Noehr A, Bonde C.

Case Reports Plast Surg Hand Surg. 2021 Nov 14;8(1):181-185. doi: 10.1080/23320885.2021.2002154. eCollection 2021.

PMID: 34790837 Free PMC article.


High prevalence of methicillin-resistant Staphylococcus aureus, Giardia, and Blastocystis in asymptomatic Syrian asylum seekers in Denmark during 2016 through 2018.

Eiset AH, Stensvold CR, Fuursted K, Nielsen HV, Wejse C.

J Migr Health. 2020 Dec 5;1-2:100016. doi: 10.1016/j.jmh.2020.100016. eCollection 2020.

PMID: 34405169 Free PMC article.


Zoonotic pathogens in wild muskoxen (Ovibos moschatus) and domestic sheep (Ovis aries) from Greenland.

Berg RPKD, Stensvold CR, Jokelainen P, Grønlund AK, Nielsen HV, Kutz S, Kapel CMO.

Vet Med Sci. 2021 Nov;7(6):2290-2302. doi: 10.1002/vms3.599. Epub 2021 Aug 14.

PMID: 34390537 Free PMC article.


Entamoeba gingivalis: epidemiology, genetic diversity and association with oral microbiota signatures in North Eastern Tanzania.

Stensvold CR, Nielsen M, Baraka V, Lood R, Fuursted K, Nielsen HV.

J Oral Microbiol. 2021 May 19;13(1):1924598. doi: 10.1080/20002297.2021.1924598.

PMID: 34104347 Free PMC article.


Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR.

Stensvold CR, Jirků-Pomajbíková K, Tams KW, Jokelainen P, Berg RPKD, Marving E, Petersen RF, Andersen LO, Angen Ø, Nielsen HV.

Microorganisms. 2021 May 31;9(6):1189. doi: 10.3390/microorganisms9061189.

PMID: 34073014 Free PMC article.


High Diversity of Cryptosporidium Species and Subtypes Identified in Cryptosporidiosis Acquired in Sweden and Abroad.

Lebbad M, Winiecka-Krusnell J, Stensvold CR, Beser J.

Pathogens. 2021 Apr 26;10(5):523. doi: 10.3390/pathogens10050523.

PMID: 33926039 Free PMC article.


Subtyping Blastocystis in pigs and humans revealed unusual avian-specific subtype ST6 in humans in Serbia.

Süli T, Kozoderović G, Potkonjak A, Vidanović D, Tešović B, Vračar V, Miladinović Tasić N, Stensvold CR, Lalošević V.

Zoonoses Public Health. 2021 Aug;68(5):544-548. doi: 10.1111/zph.12829. Epub 2021 Apr 6.

PMID: 33822468


Genetic characterization of Blastocystis from poultry, livestock animals and humans in the southwest region of Iran-Zoonotic implications.

Salehi R, Rostami A, Mirjalali H, Stensvold CR, Haghighi A.

Transbound Emerg Dis. 2022 May;69(3):1178-1185. doi: 10.1111/tbed.14078. Epub 2021 Mar 30.

PMID: 33735537



Kärssin A, Remes N, Korge K, Viigipuu M, Stensvold CR, Gómez-Morales MA, Ludovisi A, Jokelainen P, Lassen B.

J Wildl Dis. 2021 Jan 6;57(1):116-124. doi: 10.7589/JWD-D-19-00011.

PMID: 33635991


Impact of Metronidazole Treatment and Dientamoeba Fragilis Colonization on Gut Microbiota Diversity.

Gotfred-Rasmussen H, Stensvold CR, Ingham AC, Johannesen TB, Andersen LO, Röser D, Nielsen HV.

J Pediatr Gastroenterol Nutr. 2021 Jul 1;73(1):23-29. doi: 10.1097/MPG.0000000000003096.

PMID: 33633081 Clinical Trial.


Prevalence and Clinical Manifestations of Giardia intestinalis and Other Intestinal Parasites in Children and Adults in Algeria.

Belkessa S, Ait-Salem E, Laatamna A, Houali K, Sönksen UW, Hakem A, Bouchene Z, Ghalmi F, Stensvold CR.

Am J Trop Med Hyg. 2021 Jan 18;104(3):910-916. doi: 10.4269/ajtmh.20-0187.

PMID: 33534771 Free PMC article.


Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria.

Belkessa S, Thomas-Lopez D, Houali K, Ghalmi F, Stensvold CR.

Microorganisms. 2020 Dec 28;9(1):54. doi: 10.3390/microorganisms9010054.

PMID: 33379186 Free PMC article.


Detection and Identification of Acanthamoeba and Other Nonviral Causes of Infectious Keratitis in Corneal Scrapings by Real-Time PCR and Next-Generation Sequencing-Based 16S-18S Gene Analysis.

Holmgaard DB, Barnadas C, Mirbarati SH, O'Brien Andersen L, Nielsen HV, Stensvold CR.

J Clin Microbiol. 2021 Jan 21;59(2):e02224-20. doi: 10.1128/JCM.02224-20. Print 2021 Jan 21.

PMID: 33239372 Free PMC article.


A Study on the Prevalence and Subtype Diversity of the Intestinal Protist Blastocystis sp. in a Gut-Healthy Human Population in the Czech Republic.

Lhotská Z, Jirků M, Hložková O, Brožová K, Jirsová D, Stensvold CR, Kolísko M, Jirků Pomajbíková K.

Front Cell Infect Microbiol. 2020 Oct 6;10:544335. doi: 10.3389/fcimb.2020.544335. eCollection 2020.

PMID: 33123491 Free PMC article.


Veterinary Students Have a Higher Risk of Contracting Cryptosporidiosis when Calves with High Fecal Cryptosporidium Loads Are Used for Fetotomy Exercises.

Thomas-Lopez D, Müller L, Vestergaard LS, Christoffersen M, Andersen AM, Jokelainen P, Agerholm JS, Stensvold CR.

Appl Environ Microbiol. 2020 Sep 17;86(19):e01250-20. doi: 10.1128/AEM.01250-20. Print 2020 Sep 17.

PMID: 32709724 Free PMC article.


Multistrain Probiotic Increases the Gut Microbiota Diversity in Obese Pregnant Women: Results from a Randomized, Double-Blind Placebo-Controlled Study.

Halkjær SI, de Knegt VE, Lo B, Nilas L, Cortes D, Pedersen AE, Mirsepasi-Lauridsen HC, Andersen LO, Nielsen HV, Stensvold CR, Johannesen TB, Kallemose T, Krogfelt KA, Petersen AM.

Curr Dev Nutr. 2020 May 27;4(7):nzaa095. doi: 10.1093/cdn/nzaa095. eCollection 2020 Jul.

PMID: 32617453 Free PMC article.


Asylum seekers' and Refugees' Changing Health (ARCH) study protocol: an observational study in Lebanon and Denmark to assess health implications of long-distance migration on communicable and non-communicable diseases and mental health.

Eiset AH, Aoun MP, Haddad RS, Naja WJ, Fuursted K, Nielsen HV, Stensvold CR, Nielsen MS, Gottlieb A, Frydenberg M, Wejse C.

BMJ Open. 2020 May 26;10(5):e034412. doi: 10.1136/bmjopen-2019-034412.

PMID: 32461293 Free PMC article.


Dietary Inulin and Trichuris suis Infection Promote Beneficial Bacteria Throughout the Porcine Gut.

Stolzenbach S, Myhill LJ, Andersen LO, Krych L, Mejer H, Williams AR, Nejsum P, Stensvold CR, Nielsen DS, Thamsborg SM.

Front Microbiol. 2020 Mar 4;11:312. doi: 10.3389/fmicb.2020.00312. eCollection 2020.

PMID: 32194529 Free PMC article.



Stensvold CR, Tan KSW, Clark CG.

Trends Parasitol. 2020 Mar;36(3):315-316. doi: 10.1016/ Epub 2020 Jan 27.

PMID: 32001134 Review. No abstract available.


Pre-empting Pandora's Box: Blastocystis Subtypes Revisited.

Stensvold CR, Clark CG.

Trends Parasitol. 2020 Mar;36(3):229-232. doi: 10.1016/ Epub 2020 Jan 27.

PMID: 32001133 Review.


Parasites modulate the gut-microbiome in insects: A proof-of-concept study.

Fredensborg BL, Fossdal Í Kálvalíð I, Johannesen TB, Stensvold CR, Nielsen HV, Kapel CMO.

PLoS One. 2020 Jan 14;15(1):e0227561. doi: 10.1371/journal.pone.0227561. eCollection 2020.

PMID: 31935259 Free PMC article.


Differentiation of Blastocystis and parasitic archamoebids encountered in untreated wastewater samples by amplicon-based next-generation sequencing.

Stensvold CR, Lebbad M, Hansen A, Beser J, Belkessa S, O'Brien Andersen L, Clark CG.

Parasite Epidemiol Control. 2019 Dec 21;9:e00131. doi: 10.1016/j.parepi.2019.e00131. eCollection 2020 May.

PMID: 31909230 Free PMC article.


Molecular Subtyping of Blastocystis from Diverse Animals in the United Arab Emirates.

AbuOdeh R, Ezzedine S, Madkour M, Stensvold CR, Samie A, Nasrallah G, AlAbsi E, ElBakri A.

Protist. 2019 Nov;170(5):125679. doi: 10.1016/j.protis.2019.125679. Epub 2019 Aug 27.

PMID: 31580985


Evaluation of the NovaLisa™ Leishmania Infantum IgG ELISA in A Reference Diagnostic Laboratory in A Non-Endemic Country.

Stensvold CR, Høst AV, Belkessa S, Nielsen HV.

Antibodies (Basel). 2019 Feb 27;8(1):20. doi: 10.3390/antib8010020.

PMID: 31544826 Free PMC article.


Plasmodium cynomolgi as Cause of Malaria in Tourist to Southeast Asia, 2018.

Hartmeyer GN, Stensvold CR, Fabricius T, Marmolin ES, Hoegh SV, Nielsen HV, Kemp M, Vestergaard LS.

Emerg Infect Dis. 2019 Oct;25(10):1936-1939. doi: 10.3201/eid2510.190448.

PMID: 31538931 Free PMC article.


Tart Cherry Concentrate Does Not Alter the Gut Microbiome, Glycaemic Control or Systemic Inflammation in a Middle-Aged Population.

Lear R, O'Leary M, O'Brien Andersen L, Holt CC, Stensvold CR, van der Giezen M, Bowtell JL.

Nutrients. 2019 May 13;11(5):1063. doi: 10.3390/nu11051063.

PMID: 31085979 Free PMC article. Clinical Trial.


Autochthonous Angiostrongylus vasorum in Finland.

Tiškina V, Lindqvist EL, Blomqvist AC, Orav M, Stensvold CR, Jokelainen P.

Vet Rec Open. 2019 Mar 17;6(1):e000314. doi: 10.1136/vetreco-2018-000314. eCollection 2019.

PMID: 30997112 Free PMC article.


Seroprevalence of Toxoplasma gondii in domestic pigs, sheep, cattle, wild boars, and moose in the Nordic-Baltic region: A systematic review and meta-analysis.

Olsen A, Berg R, Tagel M, Must K, Deksne G, Enemark HL, Alban L, Johansen MV, Nielsen HV, Sandberg M, Lundén A, Stensvold CR, Pires SM, Jokelainen P.

Parasite Epidemiol Control. 2019 Mar 4;5:e00100. doi: 10.1016/j.parepi.2019.e00100. eCollection 2019 May.

PMID: 30906889 Free PMC article. Review.


Pinning down the role of common luminal intestinal parasitic protists in human health and disease - status and challenges.

Stensvold CR.

Parasitology. 2019 May;146(6):695-701. doi: 10.1017/S0031182019000039. Epub 2019 Feb 8.

PMID: 30732665 Review.


Development and evaluation of molecular tools for detecting and differentiating intestinal amoebae in healthy individuals.

Chihi A, Stensvold CR, Ben-Abda I, Ben-Romdhane R, Aoun K, Siala E, Bouratbine A.

Parasitology. 2019 May;146(6):821-827. doi: 10.1017/S0031182018002196. Epub 2019 Jan 14.

PMID: 30638175


Stability and resilience of the intestinal microbiota in children in daycare - a 12 month cohort study.

Mortensen MS, Hebbelstrup Jensen B, Williams J, Brejnrod AD, O'Brien Andersen L, Röser D, Andreassen BU, Petersen AM, Stensvold CR, Sørensen SJ, Krogfelt KA.

BMC Microbiol. 2018 Dec 22;18(1):223. doi: 10.1186/s12866-018-1367-5.

PMID: 30579350 Free PMC article.


Mucosal Barrier and Th2 Immune Responses Are Enhanced by Dietary Inulin in Pigs Infected With Trichuris suis.

Myhill LJ, Stolzenbach S, Hansen TVA, Skovgaard K, Stensvold CR, Andersen LO, Nejsum P, Mejer H, Thamsborg SM, Williams AR.

Front Immunol. 2018 Nov 9;9:2557. doi: 10.3389/fimmu.2018.02557. eCollection 2018.

PMID: 30473696 Free PMC article.


[Fasciola hepatica infection acquired in Denmark].

Stensvold CR, Tilma J, Tilma J, Tilma K.

Ugeskr Laeger. 2018 Oct 29;180(44):V06180395.

PMID: 30375969 Danish.


Occurrence and subtype distribution of Blastocystis sp. in humans, dogs and cats sharing household in northern Spain and assessment of zoonotic transmission risk.

Paulos S, Köster PC, de Lucio A, Hernández-de-Mingo M, Cardona GA, Fernández-Crespo JC, Stensvold CR, Carmena D.

Zoonoses Public Health. 2018 Dec;65(8):993-1002. doi: 10.1111/zph.12522. Epub 2018 Sep 9.

PMID: 30198123


Evaluating rodent experimental models for studies of Blastocystis ST1.

Růžková J, Květoňová D, Jirků M, Lhotská Z, Stensvold CR, Parfrey LW, Jirků Pomajbíková K.

Exp Parasitol. 2018 Aug;191:55-61. doi: 10.1016/j.exppara.2018.06.009. Epub 2018 Jun 28.

PMID: 29959915


Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.

Krogsgaard LR, Andersen LO', Johannesen TB, Engsbro AL, Stensvold CR, Nielsen HV, Bytzer P.

Clin Transl Gastroenterol. 2018 Jun 19;9(6):161. doi: 10.1038/s41424-018-0027-2.

PMID: 29915224 Free PMC article.


Adventure tourism and schistosomiasis: serology and clinical findings in a group of Danish students after white-water rafting in Uganda.

Röser D, Bjerrum S, Helleberg M, Nielsen HV, David KP, Thybo S, Stensvold CR.

JMM Case Rep. 2018 Feb 2;5(4):e005141. doi: 10.1099/jmmcr.0.005141. eCollection 2018 Apr.

PMID: 29868173 Free PMC article.


Associations between Gut Microbiota and Common Luminal Intestinal Parasites.

Stensvold CR, van der Giezen M.

Trends Parasitol. 2018 May;34(5):369-377. doi: 10.1016/ Epub 2018 Mar 19.

PMID: 29567298 Review.


Evaluation of a PCR Method for Detection of Entamoeba polecki, with an Overview of Its Molecular Epidemiology.

Stensvold CR, Winiecka-Krusnell J, Lier T, Lebbad M.

J Clin Microbiol. 2018 Apr 25;56(5):e00154-18. doi: 10.1128/JCM.00154-18. Print 2018 May.

PMID: 29491022 Free PMC article.


Prevalence and subtype identification of Blastocystis isolated from humans in Ahvaz, Southwestern Iran.

Salehi R, Haghighi A, Stensvold CR, Kheirandish F, Azargashb E, Raeghi S, Kohansal C, Bahrami F.

Gastroenterol Hepatol Bed Bench. 2017 Summer;10(3):235-241.

PMID: 29118941 Free PMC article.


Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species.

Carrillo-Ávila JA, Serrano-García ML, Fernández-Parra J, Sorlózano-Puerto A, Navarro-Marí JM, Stensvold CR, Gutiérrez-Fernández J.

J Med Microbiol. 2017 Oct;66(10):1436-1442. doi: 10.1099/jmm.0.000603. Epub 2017 Oct 4.

PMID: 28972465


Blastocystis subtypes detected in long-tailed macaques in Thailand-Further evidence of cryptic host specificity.

Vaisusuk K, Saijuntha W, Sedlak S, Thanchomnang T, Pilap W, Suksavate W, Stensvold CR, Tantrawatpan C.

Acta Trop. 2018 Aug;184:78-82. doi: 10.1016/j.actatropica.2017.09.002. Epub 2017 Sep 5.

PMID: 28887122


Distribution and phylogenetic analysis of Blastocystis sp. subtypes isolated from IBD patients and healthy individuals in Iran.

Mirjalali H, Abbasi MR, Naderi N, Hasani Z, Mirsamadi ES, Stensvold CR, Balaii H, Asadzadeh Aghdaei H, Zali MR.

Eur J Clin Microbiol Infect Dis. 2017 Dec;36(12):2335-2342. doi: 10.1007/s10096-017-3065-x. Epub 2017 Jul 25.

PMID: 28741097


Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: Western Europe.

Laranjo-González M, Devleesschauwer B, Trevisan C, Allepuz A, Sotiraki S, Abraham A, Afonso MB, Blocher J, Cardoso L, Correia da Costa JM, Dorny P, Gabriël S, Gomes J, Gómez-Morales MÁ, Jokelainen P, Kaminski M, Krt B, Magnussen P, Robertson LJ, Schmidt V, Schmutzhard E, Smit GSA, Šoba B, Stensvold CR, Starič J, Troell K, Rataj AV, Vieira-Pinto M, Vilhena M, Wardrop NA, Winkler AS, Dermauw V.

Parasit Vectors. 2017 Jul 21;10(1):349. doi: 10.1186/s13071-017-2280-8.

PMID: 28732550 Free PMC article. Review.


Immune responses and parasitological observations induced during probiotic treatment with medicinal Trichuris suis ova in a healthy volunteer.

Williams AR, Dige A, Rasmussen TK, Hvas CL, Dahlerup JF, Iversen L, Stensvold CR, Agnholt J, Nejsum P.

Immunol Lett. 2017 Aug;188:32-37. doi: 10.1016/j.imlet.2017.06.002. Epub 2017 Jun 8.

PMID: 28602842


The disease burden of congenital toxoplasmosis in Denmark, 2014.

Nissen J, Jokelainen P, Stensvold CR, Trevisan C, Fuchs J, Burgdorf KS, Nielsen HV, Pires SM.

PLoS One. 2017 May 30;12(5):e0178282. doi: 10.1371/journal.pone.0178282. eCollection 2017.

PMID: 28558051 Free PMC article.


Retrospective PCR-based species identification of Leishmania in two patients with visceral leishmaniasis in Serbia.

Dakić Z, Nielsen HV, Pavlović M, Poluga J, Stevanović G, Lavadinović L, Milošević B, Pelemiš M, Urošević A, Jovanović S, Stensvold CR.

JMM Case Rep. 2016 Oct 20;3(5):e005063. doi: 10.1099/jmmcr.0.005063. eCollection 2016 Oct.

PMID: 28348785 Free PMC article.


Fatal primary amoebic meningoencephalitis in a Norwegian tourist returning from Thailand.

Stubhaug TT, Reiakvam OM, Stensvold CR, Hermansen NO, Holberg-Petersen M, Antal EA, Gaustad K, Førde IS, Heger B.

JMM Case Rep. 2016 Jun 25;3(3):e005042. doi: 10.1099/jmmcr.0.005042. eCollection 2016 Jun.

PMID: 28348761 Free PMC article.


Dientamoeba fragilis, a Commensal in Children in Danish Day Care Centers.

Jokelainen P, Hebbelstrup Jensen B, Andreassen BU, Petersen AM, Röser D, Krogfelt KA, Nielsen HV, Stensvold CR.

J Clin Microbiol. 2017 Jun;55(6):1707-1713. doi: 10.1128/JCM.00037-17. Epub 2017 Mar 22.

PMID: 28330885 Free PMC article.


Inter- and intra-subtype variation of Blastocystis subtypes isolated from diarrheic and non-diarrheic patients in Iran.

Alinaghizade A, Mirjalali H, Mohebali M, Stensvold CR, Rezaeian M.

Infect Genet Evol. 2017 Jun;50:77-82. doi: 10.1016/j.meegid.2017.02.016. Epub 2017 Feb 24.

PMID: 28238960


Molecular Identification and Subtype Analysis of Blastocystis.

Stensvold CR, Clark CG.

Curr Protoc Microbiol. 2016 Nov 18;43:20A.2.1-20A.2.10. doi: 10.1002/cpmc.17.

PMID: 27858971


Blastocystis: Isolation, Xenic Cultivation, and Cryopreservation.

Clark CG, Stensvold CR.

Curr Protoc Microbiol. 2016 Nov 18;43:20A.1.1-20A.1.8. doi: 10.1002/cpmc.18.

PMID: 27858970


Blastocystis Mitochondrial Genomes Appear to Show Multiple Independent Gains and Losses of Start and Stop Codons.

Jacob AS, Andersen LO, Bitar PP, Richards VP, Shah S, Stanhope MJ, Stensvold CR, Clark CG.

Genome Biol Evol. 2016 Dec 14;8(11):3340-3350. doi: 10.1093/gbe/evw255.

PMID: 27811175 Free PMC article.


Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution.

Cacciò SM, Sannella AR, Bruno A, Stensvold CR, David EB, Guimarães S, Manuali E, Magistrali C, Mahdad K, Beaman M, Maserati R, Tosini F, Pozio E.

Int J Parasitol. 2016 Nov;46(12):793-798. doi: 10.1016/j.ijpara.2016.07.002. Epub 2016 Aug 21.

PMID: 27555056


Editorial - PAR INT - special issue on Blastocystis.

Dogruman-Al F, Stensvold CR, Yoshikawa H.

Parasitol Int. 2016 Dec;65(6 Pt B):749. doi: 10.1016/j.parint.2016.08.014.

PMID: 27742000 No abstract available.


Enteroaggregative Escherichia coli in Daycare-A 1-Year Dynamic Cohort Study.

Hebbelstrup Jensen B, Stensvold CR, Struve C, Olsen KE, Scheutz F, Boisen N, Röser D, Andreassen BU, Nielsen HV, Schønning K, Petersen AM, Krogfelt KA.

Front Cell Infect Microbiol. 2016 Jul 13;6:75. doi: 10.3389/fcimb.2016.00075. eCollection 2016.

PMID: 27468409 Free PMC article.


Current status of Blastocystis: A personal view.

Stensvold CR, Clark CG.

Parasitol Int. 2016 Dec;65(6 Pt B):763-771. doi: 10.1016/j.parint.2016.05.015. Epub 2016 May 28.

PMID: 27247124 Review.


Associations between common intestinal parasites and bacteria in humans as revealed by qPCR.

O'Brien Andersen L, Karim AB, Roager HM, Vigsnæs LK, Krogfelt KA, Licht TR, Stensvold CR.

Eur J Clin Microbiol Infect Dis. 2016 Sep;35(9):1427-31. doi: 10.1007/s10096-016-2680-2. Epub 2016 May 26.

PMID: 27230509


Epidemiological Aspects of Blastocystis Colonization in Children in Ilero, Nigeria.

Poulsen CS, Efunshile AM, Nelson JA, Stensvold CR.

Am J Trop Med Hyg. 2016 Jul 6;95(1):175-9. doi: 10.4269/ajtmh.16-0074. Epub 2016 May 2.

PMID: 27139454 Free PMC article.


Systematic review on Endolimax nana: A less well studied intestinal ameba.

Poulsen CS, Stensvold CR.

Trop Parasitol. 2016 Jan-Jun;6(1):8-29. doi: 10.4103/2229-5070.175077.

PMID: 26998431 Free PMC article. Review.


[The first case of Hypoderma tarandi-associated human myiasis in Greenland].

Bangsø J, Thøgersen KF, Nejsum P, Stensvold CR.

Ugeskr Laeger. 2016 Jan 4;178(1):V10150796.

PMID: 26750192 Danish.


Blastocystis in Health and Disease: Are We Moving from a Clinical to a Public Health Perspective?

Andersen LO, Stensvold CR.

J Clin Microbiol. 2016 Mar;54(3):524-8. doi: 10.1128/JCM.02520-15. Epub 2015 Dec 16.

PMID: 26677249 Free PMC article. Review.


Prevalence and subtype distribution of Blastocystis in healthy individuals in Sharjah, United Arab Emirates.

AbuOdeh R, Ezzedine S, Samie A, Stensvold CR, ElBakri A.

Infect Genet Evol. 2016 Jan;37:158-62. doi: 10.1016/j.meegid.2015.11.021. Epub 2015 Nov 27.

PMID: 26611823


Gut Microbiota Dysbiosis as Risk and Premorbid Factors of IBD and IBS Along the Childhood-Adulthood Transition.

Putignani L, Del Chierico F, Vernocchi P, Cicala M, Cucchiara S, Dallapiccola B; Dysbiotrack Study Group.

Inflamm Bowel Dis. 2016 Feb;22(2):487-504. doi: 10.1097/MIB.0000000000000602.

PMID: 26588090 Review.


Mitochondrial Genome Analyses Suggest Multiple Trichuris Species in Humans, Baboons, and Pigs from Different Geographical Regions.

Hawash MB, Andersen LO, Gasser RB, Stensvold CR, Nejsum P.

PLoS Negl Trop Dis. 2015 Sep 14;9(9):e0004059. doi: 10.1371/journal.pntd.0004059. eCollection 2015.

PMID: 26367282 Free PMC article.


Are Human Intestinal Eukaryotes Beneficial or Commensals?

Lukeš J, Stensvold CR, Jirků-Pomajbíková K, Wegener Parfrey L.

PLoS Pathog. 2015 Aug 13;11(8):e1005039. doi: 10.1371/journal.ppat.1005039. eCollection 2015 Aug.

PMID: 26270819 Free PMC article. No abstract available.


A retrospective metagenomics approach to studying Blastocystis.

Andersen LO, Bonde I, Nielsen HB, Stensvold CR.

FEMS Microbiol Ecol. 2015 Jul;91(7):fiv072. doi: 10.1093/femsec/fiv072. Epub 2015 Jun 29.

PMID: 26130823


Molecular Detection of the Carriage Rate of Four Intestinal Protozoa with Real-Time Polymerase Chain Reaction: Possible Overdiagnosis of Entamoeba histolytica in Nigeria.

Efunshile MA, Ngwu BA, Kurtzhals JA, Sahar S, König B, Stensvold CR.

Am J Trop Med Hyg. 2015 Aug;93(2):257-62. doi: 10.4269/ajtmh.14-0781. Epub 2015 Jun 22.

PMID: 26101274 Free PMC article.


Cryptosporidium infections in Denmark, 2010-2014.

Stensvold CR, Ethelberg S, Hansen L, Sahar S, Voldstedlund M, Kemp M, Hartmeyer GN, Otte E, Engsbro AL, Nielsen HV, Mølbak K.

Dan Med J. 2015 May;62(5):A5086.

PMID: 26050832


Babesia spp. and other pathogens in ticks recovered from domestic dogs in Denmark.

Stensvold CR, Al Marai D, Andersen LO, Krogfelt KA, Jensen JS, Larsen KS, Nielsen HV.

Parasit Vectors. 2015 May 8;8:262. doi: 10.1186/s13071-015-0843-0.

PMID: 25951937 Free PMC article.


Development and Application of a Blastocystis Subtype-Specific PCR Assay Reveals that Mixed-Subtype Infections Are Common in a Healthy Human Population.

Scanlan PD, Stensvold CR, Cotter PD.

Appl Environ Microbiol. 2015 Jun 15;81(12):4071-6. doi: 10.1128/AEM.00520-15. Epub 2015 Apr 3.

PMID: 25841010 Free PMC article.


Development and Application of a gp60-Based Typing Assay for Cryptosporidium viatorum.

Stensvold CR, Elwin K, Winiecka-Krusnell J, Chalmers RM, Xiao L, Lebbad M.

J Clin Microbiol. 2015 Jun;53(6):1891-7. doi: 10.1128/JCM.00313-15. Epub 2015 Apr 1.

PMID: 25832304 Free PMC article.


Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis.

Bremer Christensen C, Soelberg J, Stensvold CR, Jäger AK.

J Ethnopharmacol. 2015 Nov 4;174:569-75. doi: 10.1016/j.jep.2015.03.006. Epub 2015 Mar 13.

PMID: 25773490


Laboratory diagnosis of Blastocystis spp.

Stensvold CR.

Trop Parasitol. 2015 Jan-Jun;5(1):3-5. doi: 10.4103/2229-5070.149885.

PMID: 25709945 Free PMC article. No abstract available.


Prevalence and subtype analysis of Blastocystis in healthy Indian individuals.

Pandey PK, Verma P, Marathe N, Shetty S, Bavdekar A, Patole MS, Stensvold CR, Shouche YS.

Infect Genet Evol. 2015 Apr;31:296-9. doi: 10.1016/j.meegid.2015.02.012. Epub 2015 Feb 17.

PMID: 25701123


History of antimicrobial use and the risk of Dientamoeba fragilis infection.

Röser D, Simonsen J, Nielsen HV, Stensvold CR, Mølbak K.

Eur J Clin Microbiol Infect Dis. 2015 Jun;34(6):1145-51. doi: 10.1007/s10096-015-2334-9. Epub 2015 Feb 7.

PMID: 25663130


Epidemiological study of the association between malaria and helminth infections in Nigeria.

Efunshile AM, Olawale T, Stensvold CR, Kurtzhals JA, König B.

Am J Trop Med Hyg. 2015 Mar;92(3):578-82. doi: 10.4269/ajtmh.14-0548. Epub 2015 Jan 26.

PMID: 25624401 Free PMC article.


[Dientamoeba fragilis infection as cause of severe abdominal discomfort and flatulence].

Halkjær S, Stensvold CR, Petersen AM.

Ugeskr Laeger. 2015 Jan 26;177(2A):30-1.

PMID: 25612954 Danish.


The prevalence of intestinal parasites is not greater among individuals with irritable bowel syndrome: a population-based case-control study.

Krogsgaard LR, Engsbro AL, Stensvold CR, Nielsen HV, Bytzer P.

Clin Gastroenterol Hepatol. 2015 Mar;13(3):507-513.e2. doi: 10.1016/j.cgh.2014.07.065. Epub 2014 Sep 16.

PMID: 25229421


The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota.

Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HG, De Vos WM, O'Toole PW, Cotter PD.

FEMS Microbiol Ecol. 2014 Oct;90(1):326-30. doi: 10.1111/1574-6941.12396. Epub 2014 Sep 15.

PMID: 25077936


Low host-specific Enterocytozoon bieneusi genotype BEB6 is common in Swedish lambs.

Stensvold CR, Beser J, Ljungström B, Troell K, Lebbad M.

Vet Parasitol. 2014 Sep 15;205(1-2):371-4. doi: 10.1016/j.vetpar.2014.06.010. Epub 2014 Jun 14.

PMID: 24986434


Current status of epidemiology and diagnosis of human sarcocystosis.

Poulsen CS, Stensvold CR.

J Clin Microbiol. 2014 Oct;52(10):3524-30. doi: 10.1128/JCM.00955-14. Epub 2014 Apr 23.

PMID: 24759707 Free PMC article. Review.


High applicability of a novel method for gp60-based subtyping of Cryptosporidium meleagridis.

Stensvold CR, Beser J, Axén C, Lebbad M.

J Clin Microbiol. 2014 Jul;52(7):2311-9. doi: 10.1128/JCM.00598-14. Epub 2014 Apr 16.

PMID: 24740082 Free PMC article.


Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.

Verweij JJ, Stensvold CR.

Clin Microbiol Rev. 2014 Apr;27(2):371-418. doi: 10.1128/CMR.00122-13.

PMID: 24696439 Free PMC article. Review.


Metronidazole therapy for treating dientamoebiasis in children is not associated with better clinical outcomes: a randomized, double-blinded and placebo-controlled clinical trial.

Röser D, Simonsen J, Stensvold CR, Olsen KE, Bytzer P, Nielsen HV, Mølbak K.

Clin Infect Dis. 2014 Jun;58(12):1692-9. doi: 10.1093/cid/ciu188. Epub 2014 Mar 18.

PMID: 24647023 Clinical Trial.


Transmission of Dientamoeba fragilis: pinworm or cysts?

Clark CG, Röser D, Stensvold CR.

Trends Parasitol. 2014 Mar;30(3):136-40. doi: 10.1016/ Epub 2014 Jan 31.

PMID: 24492020 Review.


Genetic diversity of Pneumocystis jirovecii in colonized Cuban infants and toddlers.

Monroy-Vaca EX, de Armas Y, Illnait-Zaragozí MT, Diaz R, Toraño G, Vega D, Alvarez-Lam I, Calderón EJ, Stensvold CR.

Infect Genet Evol. 2014 Mar;22:60-6. doi: 10.1016/j.meegid.2013.12.024. Epub 2014 Jan 9.

PMID: 24412726


Prevalence, incidence, and risk factors of intestinal parasites in Danish primary care patients with irritable bowel syndrome.

Engsbro AL, Stensvold CR, Vedel Nielsen H, Bytzer P.

Scand J Infect Dis. 2014 Mar;46(3):204-9. doi: 10.3109/00365548.2013.861609. Epub 2013 Dec 17.

PMID: 24344761


Prevalence and genotype distribution of Pneumocystis jirovecii in Cuban infants and toddlers with whooping cough.

Monroy-Vaca EX, de Armas Y, Illnait-Zaragozí MT, Toraño G, Diaz R, Vega D, Alvarez-Lam I, Calderón EJ, Stensvold CR.

J Clin Microbiol. 2014 Jan;52(1):45-51. doi: 10.1128/JCM.02381-13. Epub 2013 Oct 16.

PMID: 24131683 Free PMC article.


Blastocystis: getting to grips with our guileful guest.

Scanlan PD, Stensvold CR.

Trends Parasitol. 2013 Nov;29(11):523-9. doi: 10.1016/ Epub 2013 Sep 27.

PMID: 24080063


Anisakiasis mistaken for dientamoebiasis?

Röser D, Stensvold CR.

Clin Infect Dis. 2013 Nov;57(10):1500. doi: 10.1093/cid/cit543. Epub 2013 Aug 20.

PMID: 23965282 No abstract available.


Blastocystis: Genetic diversity and molecular methods for diagnosis and epidemiology.

Stensvold CR.

Trop Parasitol. 2013 Jan;3(1):26-34. doi: 10.4103/2229-5070.113896.

PMID: 23961438 Free PMC article.


Blastocystis subtypes detected in humans and animals from Colombia.

Ramírez JD, Sánchez LV, Bautista DC, Corredor AF, Flórez AC, Stensvold CR.

Infect Genet Evol. 2014 Mar;22:223-8. doi: 10.1016/j.meegid.2013.07.020. Epub 2013 Jul 22.

PMID: 23886615


Genetic diversity of blastocystis in livestock and zoo animals.

Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG.

Protist. 2013 Jul;164(4):497-509. doi: 10.1016/j.protis.2013.05.003. Epub 2013 Jun 14.

PMID: 23770574


Limited intra-genetic diversity in Dientamoeba fragilis housekeeping genes.

Stensvold CR, Clark CG, Röser D.

Infect Genet Evol. 2013 Aug;18:284-6. doi: 10.1016/j.meegid.2013.05.003. Epub 2013 May 13.

PMID: 23681023


Dientamoeba fragilis in Denmark: epidemiological experience derived from four years of routine real-time PCR.

Röser D, Simonsen J, Nielsen HV, Stensvold CR, Mølbak K.

Eur J Clin Microbiol Infect Dis. 2013 Oct;32(10):1303-10. doi: 10.1007/s10096-013-1880-2. Epub 2013 Apr 24.

PMID: 23609513


Diversity and distribution of Blastocystis sp. subtypes in non-human primates.

Alfellani MA, Jacob AS, Perea NO, Krecek RC, Taner-Mulla D, Verweij JJ, Levecke B, Tannich E, Clark CG, Stensvold CR.

Parasitology. 2013 Jul;140(8):966-71. doi: 10.1017/S0031182013000255. Epub 2013 Apr 8.

PMID: 23561720


Recent developments in Blastocystis research.

Clark CG, van der Giezen M, Alfellani MA, Stensvold CR.

Adv Parasitol. 2013;82:1-32. doi: 10.1016/B978-0-12-407706-5.00001-0.

PMID: 23548084 Review.


Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection.

Petersen AM, Stensvold CR, Mirsepasi H, Engberg J, Friis-Møller A, Porsbo LJ, Hammerum AM, Nordgaard-Lassen I, Nielsen HV, Krogfelt KA.

Scand J Gastroenterol. 2013 May;48(5):638-9. doi: 10.3109/00365521.2013.780094. Epub 2013 Mar 25.

PMID: 23528075 No abstract available.


Comparison of six simple methods for extracting ribosomal and mitochondrial DNA from Toxocara and Toxascaris nematodes.

Mikaeili F, Kia EB, Sharbatkhori M, Sharifdini M, Jalalizand N, Heidari Z, Zarei Z, Stensvold CR, Mirhendi H.

Exp Parasitol. 2013 Jun;134(2):155-9. doi: 10.1016/j.exppara.2013.02.008. Epub 2013 Mar 13.

PMID: 23499880


Azole resistance in Aspergillus fumigatus from bronchoalveolar lavage fluid samples of patients with chronic diseases.

Zhao Y, Stensvold CR, Perlin DS, Arendrup MC.

J Antimicrob Chemother. 2013 Jul;68(7):1497-504. doi: 10.1093/jac/dkt071. Epub 2013 Mar 5.

PMID: 23463213 Free PMC article.


Waiting for the human intestinal Eukaryotome.

Andersen LO, Vedel Nielsen H, Stensvold CR.

ISME J. 2013 Jul;7(7):1253-5. doi: 10.1038/ismej.2013.21. Epub 2013 Feb 14.

PMID: 23407309 Free PMC article. No abstract available.


Is supplementary bead beating for DNA extraction from nematode eggs by use of the NucliSENS easyMag protocol necessary?

Andersen LO, Röser D, Nejsum P, Nielsen HV, Stensvold CR.

J Clin Microbiol. 2013 Apr;51(4):1345-7. doi: 10.1128/JCM.03353-12. Epub 2013 Jan 16.

PMID: 23325821 Free PMC article. No abstract available.


Variable geographic distribution of Blastocystis subtypes and its potential implications.

Alfellani MA, Stensvold CR, Vidal-Lapiedra A, Onuoha ES, Fagbenro-Beyioku AF, Clark CG.

Acta Trop. 2013 Apr;126(1):11-8. doi: 10.1016/j.actatropica.2012.12.011. Epub 2013 Jan 3.

PMID: 23290980


DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis.

Röser D, Nejsum P, Carlsgart AJ, Nielsen HV, Stensvold CR.

Exp Parasitol. 2013 Jan;133(1):57-61. doi: 10.1016/j.exppara.2012.10.009. Epub 2012 Oct 29.

PMID: 23116599


Comparison of sequencing (barcode region) and sequence-tagged-site PCR for Blastocystis subtyping.

Stensvold CR.

J Clin Microbiol. 2013 Jan;51(1):190-4. doi: 10.1128/JCM.02541-12. Epub 2012 Oct 31.

PMID: 23115257 Free PMC article.


Treatment of Dientamoeba fragilis in patients with irritable bowel syndrome.

Engsbro AL, Stensvold CR, Nielsen HV, Bytzer P.

Am J Trop Med Hyg. 2012 Dec;87(6):1046-52. doi: 10.4269/ajtmh.2012.11-0761. Epub 2012 Oct 22.

PMID: 23091195 Free PMC article.


Ultraviolet irradiation: An effective inactivation method of Aspergillus spp. in water for the control of waterborne nosocomial aspergillosis.

Nourmoradi H, Nikaeen M, Stensvold CR, Mirhendi H.

Water Res. 2012 Nov 15;46(18):5935-40. doi: 10.1016/j.watres.2012.08.015. Epub 2012 Aug 30.

PMID: 22985523


Blastocystis: to treat or not to treat...but how?

Engsbro AL, Stensvold CR.

Clin Infect Dis. 2012 Nov 15;55(10):1431-2. doi: 10.1093/cid/cis699. Epub 2012 Aug 14.

PMID: 22893582 No abstract available.


Thinking Blastocystis out of the box.

Stensvold CR.

Trends Parasitol. 2012 Aug;28(8):305. doi: 10.1016/ Epub 2012 Jun 15.

PMID: 22704911 No abstract available.


Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp.

Stensvold CR, Ahmed UN, Andersen LO, Nielsen HV.

J Clin Microbiol. 2012 Jun;50(6):1847-51. doi: 10.1128/JCM.00007-12. Epub 2012 Mar 14.

PMID: 22422846 Free PMC article.


Subtype analysis of Blastocystis isolates in Swedish patients.

Forsell J, Granlund M, Stensvold CR, Clark CG, Evengård B.

Eur J Clin Microbiol Infect Dis. 2012 Jul;31(7):1689-96. doi: 10.1007/s10096-011-1416-6. Epub 2012 Feb 15.

PMID: 22350386


Short report: Molecular characterization of Blastocystis obtained from members of the indigenous Tapirapé ethnic group from the Brazilian Amazon region, Brazil.

Malheiros AF, Stensvold CR, Clark CG, Braga GB, Shaw JJ.

Am J Trop Med Hyg. 2011 Dec;85(6):1050-3. doi: 10.4269/ajtmh.2011.11-0481.

PMID: 22144442 Free PMC article.


Levels of genetic diversity vary dramatically between Blastocystis subtypes.

Stensvold CR, Alfellani M, Clark CG.

Infect Genet Evol. 2012 Mar;12(2):263-73. doi: 10.1016/j.meegid.2011.11.002. Epub 2011 Nov 17.

PMID: 22116021


Comparison of microscopy and PCR for detection of intestinal parasites in Danish patients supports an incentive for molecular screening platforms.

Stensvold CR, Nielsen HV.

J Clin Microbiol. 2012 Feb;50(2):540-1. doi: 10.1128/JCM.06012-11. Epub 2011 Nov 16.

PMID: 22090410 Free PMC article. No abstract available.


Blastocystis subtypes in symptomatic and asymptomatic family members and pets and response to therapy.

Nagel R, Cuttell L, Stensvold CR, Mills PC, Bielefeldt-Ohmann H, Traub RJ.

Intern Med J. 2012 Nov;42(11):1187-95. doi: 10.1111/j.1445-5994.2011.02626.x.

PMID: 22032439


Last of the human protists: the phylogeny and genetic diversity of Iodamoeba.

Stensvold CR, Lebbad M, Clark CG.

Mol Biol Evol. 2012 Jan;29(1):39-42. doi: 10.1093/molbev/msr238. Epub 2011 Sep 22.

PMID: 21940643


Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea.

Stensvold CR, Christiansen DB, Olsen KE, Nielsen HV.

Am J Trop Med Hyg. 2011 Jun;84(6):883-5. doi: 10.4269/ajtmh.2011.11-0005.

PMID: 21633023 Free PMC article.


Increased sampling reveals novel lineages of Entamoeba: consequences of genetic diversity and host specificity for taxonomy and molecular detection.

Stensvold CR, Lebbad M, Victory EL, Verweij JJ, Tannich E, Alfellani M, Legarraga P, Clark CG.

Protist. 2011 Jul;162(3):525-41. doi: 10.1016/j.protis.2010.11.002. Epub 2011 Feb 3.

PMID: 21295520



Stensvold CR, Nielsen HV, Petersen E.

Ugeskr Laeger. 2011 Jan 17;173(3):186-9.

PMID: 21241625 Danish.


The impact of genetic diversity in protozoa on molecular diagnostics.

Stensvold CR, Lebbad M, Verweij JJ.

Trends Parasitol. 2011 Feb;27(2):53-8. doi: 10.1016/ Epub 2010 Dec 16.

PMID: 21168365


The prevalence and clinical significance of intestinal parasites in HIV-infected patients in Denmark.

Stensvold CR, Nielsen SD, Badsberg JH, Engberg J, Friis-Møller N, Nielsen SS, Nielsen HV, Friis-Møller A.

Scand J Infect Dis. 2011 Feb;43(2):129-35. doi: 10.3109/00365548.2010.524659. Epub 2010 Oct 11.

PMID: 20936912


Identification and delineation of members of the Entamoeba complex by pyrosequencing.

Stensvold CR, Lebbad M, Verweij JJ, Jespersgaard C, von Samson-Himmelstjerna G, Nielsen SS, Nielsen HV.

Mol Cell Probes. 2010 Dec;24(6):403-6. doi: 10.1016/j.mcp.2010.07.008. Epub 2010 Aug 4.

PMID: 20691255


Blastocystis sp. subtype 2 detection during recurrence of gastrointestinal and urticarial symptoms.

Vogelberg C, Stensvold CR, Monecke S, Ditzen A, Stopsack K, Heinrich-Gräfe U, Pöhlmann C.

Parasitol Int. 2010 Sep;59(3):469-71. doi: 10.1016/j.parint.2010.03.009. Epub 2010 Apr 2.

PMID: 20363362


Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants.

Stensvold CR, Lebbad M, Clark CG.

Int J Parasitol. 2010 Jun;40(7):775-8. doi: 10.1016/j.ijpara.2010.03.003. Epub 2010 Mar 30.

PMID: 20359482


Identification of zoonotic genotypes of Giardia duodenalis.

Sprong H, Cacciò SM, van der Giessen JW; ZOOPNET network and partners.

PLoS Negl Trop Dis. 2009 Dec 1;3(12):e558. doi: 10.1371/journal.pntd.0000558.

PMID: 19956662 Free PMC article.


Eradication of Blastocystis carriage with antimicrobials: reality or delusion?

Stensvold CR, Smith HV, Nagel R, Olsen KE, Traub RJ.

J Clin Gastroenterol. 2010 Feb;44(2):85-90. doi: 10.1097/MCG.0b013e3181bb86ba.

PMID: 19834337 Review.


[Blastocystis--an enigmatic parasite].

Stensvold CR, Arendrup MC, Nielsen HV, Mølbak K.

Ugeskr Laeger. 2009 Aug 17;171(34):2388-90.

PMID: 19732521 Danish.


Seroprevalence of human toxocariasis in Denmark.

Stensvold CR, Skov J, Møller LN, Jensen PM, Kapel CM, Petersen E, Nielsen HV.

Clin Vaccine Immunol. 2009 Sep;16(9):1372-3. doi: 10.1128/CVI.00234-09. Epub 2009 Jul 29.

PMID: 19641098 Free PMC article.


Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite.

Stensvold CR, Lewis HC, Hammerum AM, Porsbo LJ, Nielsen SS, Olsen KE, Arendrup MC, Nielsen HV, Mølbak K.

Epidemiol Infect. 2009 Nov;137(11):1655-63. doi: 10.1017/S0950268809002672. Epub 2009 Apr 27.

PMID: 19393117


Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients.

Rene BA, Stensvold CR, Badsberg JH, Nielsen HV.

Am J Trop Med Hyg. 2009 Apr;80(4):588-92.

PMID: 19346381


A foodborne outbreak of Cryptosporidium hominis infection.

Ethelberg S, Lisby M, Vestergaard LS, Enemark HL, Olsen KE, Stensvold CR, Nielsen HV, Porsbo LJ, Plesner AM, Mølbak K.

Epidemiol Infect. 2009 Mar;137(3):348-56. doi: 10.1017/S0950268808001817. Epub 2009 Feb 9.

PMID: 19134228


Pursuing the clinical significance of Blastocystis--diagnostic limitations.

Stensvold CR, Nielsen HV, Mølbak K, Smith HV.

Trends Parasitol. 2009 Jan;25(1):23-9. doi: 10.1016/ Epub 2008 Nov 13.

PMID: 19013108 Review.


Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype.

Stensvold CR, Alfellani MA, Nørskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG.

Int J Parasitol. 2009 Mar;39(4):473-9. doi: 10.1016/j.ijpara.2008.07.006. Epub 2008 Aug 9.

PMID: 18755193


Symptomatic infection with Blastocystis sp. subtype 8 successfully treated with trimethoprim-sulfamethoxazole.

Stensvold CR, Arendrup MC, Nielsen HV, Bada A, Thorsen S.

Ann Trop Med Parasitol. 2008 Apr;102(3):271-4. doi: 10.1179/136485908X278847.

PMID: 18348782 No abstract available.


Molecular epidemiology of Blastocystis infections in Turkey.

Ozyurt M, Kurt O, Mølbak K, Nielsen HV, Haznedaroglu T, Stensvold CR.

Parasitol Int. 2008 Sep;57(3):300-6. doi: 10.1016/j.parint.2008.01.004. Epub 2008 Feb 13.

PMID: 18337161


Improvement of PCR for detection of Opisthorchis viverrini DNA in human stool samples.

Duenngai K, Sithithaworn P, Rudrappa UK, Iddya K, Laha T, Stensvold CR, Strandgaard H, Johansen MV.

J Clin Microbiol. 2008 Jan;46(1):366-8. doi: 10.1128/JCM.01323-07. Epub 2007 Nov 14.

PMID: 18003810 Free PMC article.


Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study.

Stensvold CR, Arendrup MC, Jespersgaard C, Mølbak K, Nielsen HV.

Diagn Microbiol Infect Dis. 2007 Nov;59(3):303-7. doi: 10.1016/j.diagmicrobio.2007.06.003. Epub 2007 Oct 29.

PMID: 17913433


A case of trichinellosis in Denmark, imported from Poland, June 2007.

Stensvold CR, Nielsen HV, Mølbak K.

Euro Surveill. 2007 Aug 9;12(8):E070809.3. doi: 10.2807/esw.12.32.03248-en.

PMID: 17868552 No abstract available.


The prevalence of Dientamoeba fragilis in patients with suspected enteroparasitic disease in a metropolitan area in Denmark.

Stensvold CR, Arendrup MC, Mølbak K, Nielsen HV.

Clin Microbiol Infect. 2007 Aug;13(8):839-42. doi: 10.1111/j.1469-0691.2007.01760.x.

PMID: 17610603


Outbreak of severe gastroenteritis with multiple aetiologies caused by contaminated drinking water in Denmark, January 2007.

Vestergaard LS, Olsen KE, Stensvold R, Böttiger BE, Adelhardt M, Lisby M, Mørk L, Mølbak K.

Euro Surveill. 2007 Mar 29;12(3):E070329.1. doi: 10.2807/esw.12.13.03164-en.

PMID: 17439795 No abstract available.


Blastocystis: subtyping isolates using pyrosequencing technology.

Stensvold CR, Traub RJ, von Samson-Himmelstjerna G, Jespersgaard C, Nielsen HV, Thompson RC.

Exp Parasitol. 2007 Jun;116(2):111-9. doi: 10.1016/j.exppara.2006.12.002. Epub 2006 Dec 24.

PMID: 17266951


Terminology for Blastocystis subtypes--a consensus.

Stensvold CR, Suresh GK, Tan KS, Thompson RC, Traub RJ, Viscogliosi E, Yoshikawa H, Clark CG.

Trends Parasitol. 2007 Mar;23(3):93-6. doi: 10.1016/ Epub 2007 Jan 22.

PMID: 17241816


Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction.

Stensvold R, Brillowska-Dabrowska A, Nielsen HV, Arendrup MC.

J Parasitol. 2006 Oct;92(5):1081-7. doi: 10.1645/GE-840R.1.

PMID: 17152954


Evaluation of PCR based coprodiagnosis of human opisthorchiasis.

Stensvold CR, Saijuntha W, Sithithaworn P, Wongratanacheewin S, Strandgaard H, Ornbjerg N, Johansen MV.

Acta Trop. 2006 Jan;97(1):26-30. doi: 10.1016/j.actatropica.2005.08.008. Epub 2005 Oct 25.

PMID: 16253202

Book chapters:

In Molecular Microbiology: Diagnostic Principles and Practice 3rd Edition (March 2016)

Chapter 38 : Molecular Detection of Gastrointestinal Parasites 

 By Jaco J. Verweij, Alex Van Belkum, C. Rune Stensvold  

DOI 10.1128/9781555819071.ch38


The continuously expanding universe of Entamoeba
Charles Graham Clark & Christen Rune Stensvold
Chapter · January 2015
DOI: 10.1007/978-4-431-55200-0_2
In book: Amebiasis. Biology and pathogenesis of Entamoeba, Chapter: 2, Publisher: Springer, Editors: Tomoyoshi Nozaki, Alok Bhattacharya, pp.9-25

Christen Rune Stensvold
Chapter · April 2015
CRC Press
Reference - 520 Pages - 89 B/W Illustrations
ISBN 9781466568839 - CAT# K16405
In book: Biology of Foodborne Parasites (edited by Lihua Xiao, Una Ryan, Yaoyu Feng)

Endolimax nana
Casper Sahl Poulsen & Christen Rune Stensvold

Christen Rune Stensvold

Katerina Jirku-Pomajbikova & Christen Rune Stensvold

Christian Paul de la Cruz & Christen Rune Stensvold

Page updated 15 AUG 2019


  1. Dear Stensfold,
    Your blog on Blastocystis looks very nice and is informative. What article would you recommend, in which it is proven that Blastocystis is directly associated with disease?
    You mentioned in your blog that typing hasn't been extensively performed on samples from sub-Saharan Africa. If you are interested, I might know a collection of such fecal DNA that might be used for further research.

  2. Dear Robert,
    thank you for stopping by!
    I'm afraid I have no such article to recommend. There are many theories, but one thing is a fact: A large proportion of the human population is colonised by Blastocystis, and most individuals do not suffer intestinal symptoms, at least over a longer period. Blastocystis has not been associated with any outbreak, invasivness has not been successfully demonstrated, and problems with chemotherapeutic treatment makes RCT studies difficult. Having said that, our French colleagues have published genome data, and Blastocystis does appear to encode potential virulence genes.

    There are so many things, thoughts and theories to discuss, many of these have been touched upon elsewhere on this blog. Also many opportunities...

    It's always interesting to look into subtype epidemiology. Please let me know if I can be of any assistance!


  3. Hi, I just got diagnosed with having Blastocystis. I am writing to you because I thought maybe you would be interested in my symptoms.
    For Christmas and New Year's I was in Cancun, Mexico with my family. We had swam in the ocean and also in a cenote there. I have a feeling inside of me we were all infected in the Cenote. All of us have been experiencing symptoms since returning from Mexico but we all thought it to be from different causes. My situation is worse than everyone else in my family. After going to the emergency room at my local hospitals on two occasions since returning from Mexico and having X-rays, ultrasounds, blood work, CT Scan, CT with contract, angiogram, EKG, and urine tests, the doctors told me I had anxiety. The reason I went to the hospital is because I have extreme shortness of breath. I have never had this before. I never had asthma or any other symptoms. I literally woke up in the middle of the night gasping for air. Shortly after I began vomiting undigested food and acid so they said I had GERD and anxiety... I never had acidity before. I took all of the meds they gave me and nothing was helping. I had this weird feeling inside of me that was like an empty cold feeling and I just knew something wasn't right. I convinced my doctor to do stool tests in which they found the blastocysts. I had begun a metronidazole regimen right after I turned in my stool samples. I was taking it 3x a day for 7 days with no improvement at all. I did notice a slight improvement with Primal Defense probiotics. I take 3 a day but still feel shortness of breath and my gut is constantly making awful noises. My doctor has now prescribed me Nitazoxinide to take for 3 days with a refill for an additional 3 if it doesn't work. I will let you know how it goes, if you would like.

  4. Thank you, Louise, for taking your time here on the blog, and for your description of your situation. I would like to reiterate that Blastocystis is a very common parasite, also in the Western world, an asymptomatic carriage is common, at least when it comes to the common subtypes. In case you never had stool tests done (for ova and parasites or some similar test) before you went to Mexico, it's impossible to know whether you were carrying Blastocystis with you to Mexico or you only caught it out there. Your account sounds to me almost like the description of a minor outbreak, which of course would be interesting to investigate in further detail. I wonder whether your family members are carrying Blastocystis too?! Feel free to post your experience on your treatment here. There is also the Blastocystis community on Facebook where you can share your story.

    Having said that I was starting to think of something else when I read your story, namely the possibility of you having been exposed to free living amoeba of some sort... However, I'm not sure about the salinity in the cenotes there in Mexico... maybe such amoebae cannot live there at all...

  5. I am on day 4 now of the treatment and it's not working. I did happen to find a center in Australia that works with Blastocyst quite frequently. Maybe you would want to speak with them. Their treatments seem promising.

    About the salinity in the cenotes, from what I gather, it is rather high and the water is not stagnant, it apparently runs through a labyrinth of under ground caves and the cenotes are sink holes from the ground down to the cave systems.

    Here is the center in Australia:

    My daughters still have not been tested. They are charging us $450 for the tests and my doctor seems to think they must have what I have because the symptoms are very similar. Both of my daughters are now having shortness of breath, as well. We have extreme fatigue and our quality of life is drastically reduced from what it was prior to the symptoms appearing.

    I'm going to try to get my doctor to communicate with this center in Australia and get their meds over here in the US to see if they will work. From what I understand from their page, they use enema like meds to eradicate the blastocysts.

    I also found this page which has a plethora of comments from people all suffering with Blastocysts and many haven't been able to clear them from their systems:

    I sincerely hope people start taking this more seriously and no just shrugging it off. I am in pain and really suffering. I hope your research will assist in treating this protozoa nightmare.