Tuesday, July 10, 2012

Blastocystis Culture in Jones' Medium

Upon request I have now posted the protocol on one of the simplest media used for Blastocystis culture, Jones' Medium, - please go to the tab (page) "Lab Stuff".

You can read about Blastocystis culture in some of my other blog posts, use the search box or the labels feature.

Please be aware that this is for xenic culture only - i.e. culture in the presence of bacteria. It's quick, inexpensive, very reliable (at least for human samples) and isolates can be kept this way for months/years - all you need is an incubator.

Extracting DNA from cultures and using it for subtyping usually yields excellent results.

I have never tried to cryopreserve (freeze down) Blastocystis using Jones' Medium, but it is possible (at least when Robinson's Medium is used).

More reading:

Stensvold CR, Arendrup MC, Jespersgaard C, Mølbak K, & Nielsen HV (2007). Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. Diagnostic microbiology and infectious disease, 59 (3), 303-7 PMID: 17913433

And, if you are interested in culture of intestinal protists in general, why not look up

Clark CG, & Diamond LS (2002). Methods for cultivation of luminal parasitic protists of clinical importance. Clinical microbiology reviews, 15 (3), 329-41 PMID: 12097242

No comments:

Post a Comment